Precision Medicine




qPCR and RT-qPCR

Quantitative PCR (qPCR) and reverse transcription qPCR (RT-PCR) are the gold standard methods for examining gene expression due to their unparalleled sensitivity, specificity, and linear dynamic range. Therefore, qPCR is the chosen technology when aiming to quantify DNA, mRNA, microRNA and lncRNA. At PeploBio, we offer a wide range of bespoke PCR services along with TaqMan (ThermoFisher) ready solutions.


Primer Probe Design for qPCR Assay Development

Designing optimal primers and probes along with stringent evaluation testing is essential when developing qPCR assays. Differentiating between probe-based versus dye-based (SYBR Green) qPCR is considered based on the sensitivity and specificity requirements of the assay. Probe-based assays offer the additional advantage of multi-plex capability when detecting multiple targets is required.


Application of qPCR

  • SNP Genotyping

  • DNA copy number detection

  • Quantification of microRNAs in liquid biopsies

  • Quantifying multi-gene signatures in whole blood and FFPE samples

  • Gene expression analysis in cell lysates


Application of qPCR

  • Virology

  • Immunology

  • Immuno-oncology

  • Neurodegenerative and complex diseases

  • Validate results from microarray and NGS based technologies

  • Pathway analysis

  • Biomarkers signatures

  • eQTL analysis


Assay Validation

PeploBio is experienced in developing and validating qPCR-based assays for either clinical trial studies or diagnostic grade products seeking UKCA, CE IVD marking or CDTA regulatory approval. Our state-of-the-art QMS and expert team of PhD and HCPC registered scientists ensures that our sponsors have access to the necessary personnel and technologies to support their study throughout its duration.

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